Service Manuals, User Guides, Schematic Diagrams or docs for : Agilent 5991-3295EN Elastic Modulus Mapping Using the 7500 AFM - Application Note c20141020 [4]

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Keysight Technologies
Elastic Modulus Mapping
Using the 7500AFM




        Application Note




        Introduction
        The atomic force microscope (AFM) 1 has become a very important tool
        for investigating samples on the nano-scale. It combines in a unique
        way high spatial resolution with very high force sensitivity, allowing for
        mapping of elastic properties with highest spatial resolution.

        For quantitative determination of elasticity a force distance curve is
        obtained. From such a force distance curve the elastic modulus can be
        extracted. A mapping of local elastic properties is achieved by obtain-
        ing two-dimensional arrays of force distance curves. Such elasticity
        maps can be used to derive information on cellular processes. In par-
        ticular, elasticity measurements provide valuable insights into various
        dynamic cellular processes such as cell migration and cell division.

        Here, we show how the Keysight Technologies, Inc. 7500 AFM can be
        utilized to map the elastic modulus of endothelial cells.
02 | Keysight | Elastic Modulus Mapping Using the 7500AFM - Application Note


                                                                                                                            Figure 1. Topographical images.
                                                                                                                            Contact mode images of a
                                                                                                                            sample cell were made to char-
                                                                                                                            acterize cell morphology. The
                                                                                                                            left image shows an overview
                                                                                                                            (50 x 50

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